To test the Photostability upon laser irradiation, 200 L of PLGA nanoparticles encapsulating DDA-GSH-AuNCs, Nile red or fluorescein were placed in UV 96-Well microplate and irradiated with the following lasers: (364 nm, 80 mW, 248 mW/cm 2 ), (568 nm, 30 mW, 93 mW/cm 2 ) and (488 nm, 30 mW, 93 mW/cm 2 ), respectively using confocal laser scanning microscope (LSM 510 META, Carl Zeiss MicroImaging GmbH, Gttingen, Germany) for total duration of 20 minutes
Nevertheless, this effect is consistent with additional molecular mechanism by which MeHg affects the GSH antioxidant system in the developing CNS, thereby rendering the brain more susceptible to oxidants
The most frequently used centrifugation conditions are 10,000 g for 20-30 min
Millsop JW, Bhatia BK, Debbaneh M, Koo J, Liao W
How is Peptide Therapy Administered